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  • 生菜的瞬时侵染后荧光蛋白表达
    生菜的瞬时侵染后荧光蛋白表达
    Expression of Fluorescent Protein after Instant Infection of Lettuce
  • 生菜植物工厂
    生菜植物工厂
    Lettuce plant
植物生物反应器表达药用蛋白体系
随着植物基因工程技术的不断发展,利用植物表达系统作为药用蛋白(如抗体、疫苗等)的 "分子加工厂 "成为植物基因工程发展的新趋势,被视为第三代生物反应器技术。

       随着植物基因工程技术的不断发展,利用植物表达系统作为药用蛋白(如抗体、疫苗等)的"分子加工厂"成为植物基因工程发展的新趋势,被视为第三代生物反应器技术。
       经研究证实,植物表达系统具有表达活性哺乳动物蛋白的能力,并且在产品质量、成本和安全方面已经凸显其优势。植物表达药用蛋白的成本仅为微生物发酵培养的2%-10%,动物细胞培养生产成本的0.1%-1%,对生物医药行业和整个社会带来巨大的经济利益。以植物为"工厂",通过多种基因工程手段实现药用蛋白高水平、高活性表达,可为人类医疗保健事业做出的贡献不可估量。
       植物是最经济的蛋白质生产系统。种植植物所需要的仅是阳光,来自土壤或肥料的矿物质营养、水分。这一点明显优于微生物发酵和动物细胞的培养,因为微生物发酵需要昂贵的设备和大量的人力投入,而动物细胞的培养需要更为昂贵的生长培养基,而且要保持无菌的生产条件。
With the continuous development of plant genetic engineering technology, the use of plant expression system as a medicinal protein (such as antibodies, vaccines, etc.) "molecular processing plant" has become a new trend in plant genetic engineering development, is considered the third generation of bioreactor
为了不断提升惠升生物的科技创新实力,引导公司核心业务的产业提升,加强公司的高端人才队伍建设,把公司打造成中国生物医药的高端研发平台,使公司早日成为具有国际竞争力的世界一流生物医药企业,市值超千亿的超级独角兽。惠升生物诚邀各类人才加盟,并愿为各类人才提供不断发挥聪明才智的创新项目、业务培训、薪酬激励、研发环境。
In order to continuously enhance the scientific and technological innovation strength of Hui Sheng biological, to guide the company's core business to enhance the industry to strengthen the company's high-end talent team building, the company hit the Chinese creaturesMedicine, high-end research and development platform, the company as soon as possible to become internationally competitive world-class bio-pharmaceutical companies, the market value of over 100 billion super unicorn. Hui Sheng biological invites all kinds of talents to join, and is willing to provide all kinds of talent to continue to play smart and innovative projects, business training, pay incentives, research and development environment.

  • 转基因烟草抗生素筛选
    转基因烟草抗生素筛选
    Screening of Antibiotics for Transgenic Tobacco
  • 显微镜下显示烟草种子
    显微镜下显示烟草种子
    The tobacco seed is shown under a microscope
技术途径
Technical approach

利用生菜瞬时表达蛋白需要通过将目标基因插入植物表达载体,但不需要建立稳定表达蛋白的纯合品系。在对生菜叶片进行侵染后四天内即可获得表达目标蛋白的生菜表,之后将生菜叶进行收获,经过蛋白提取和纯化过程,即可获得高纯度的目标蛋白。

The use of lettuce to express proteins instantaneously requires the insertion of the target gene into the plant expression vector, but does not require the establishment of a homozygous strain that stably expresses the protein. In the four days after the infestation of lettuce leaves, the lettuce table expressing the target protein was obtained, and the lettuce leaves were harvested. After the protein extraction and purification process, the target protein with high purity could be obtained.

瞬时表达目标蛋白技术平台生产工艺流程
Instantaneous expression of target protein technology platform production process

比较优势
Comparative Advantage

  • 蛋白产量高
    High protein production:

    1)单株生菜蛋白生产量高:惠升专有平台技术使生菜瞬时生产外源蛋白量提高到了1.67g/kg,是目前所知瞬间生产系统最高的生产量;
    2)侵染效率高:整株生菜的侵染阳性率的提高到了90%以上,而其他技术侵染效率只有不到50%。

    1) High yield of single lettuce protein: high-yield platform technology to make lettuce instantaneous production of foreign protein increased to 1.67g / kg, is now known instantaneous production system the highest production capacity;
    2) High efficiency of infection: the positive rate of whole plant lettuce infection increased to more than 90%, while other technology infection efficiency of less than 50%.

     
  • 研发周期短
    R & D cycle is short

    对比传统植物分子医药1-2年的研发周期,惠升利用专有平台技术,在短短4天内就能获得目标蛋白质进行功能检测,大大缩短了产品开发时间,研发可持续性强。

    Compared with the traditional plant molecularmedicine1-2 years of research and development cycle, the use of proprietary platform technology, in just 4 days will be able to obtain the target protein for functional testing, greatly reducing the product development time, research and development sustainability.

     
  • 原料供应无季节限制
    No seasonal restrictions on raw material supply

    生菜生长周期仅为35天,且种植占地面积小,可实现封闭式管理,无土栽培,全年无限量供应。

    Lettuce growth cycle is only 35 days, and planting covers an area of small, can be closed management, soilless cultivation, unlimited supply throughout the year.

     
  • 全流程GMP操作管理
    Full Process GMP Operations Management

    解决植物种植均一性问题: 建立自动化生产流水线,并结合育种技术与自动化播种、种植、灌溉和挑选采摘为一体的植物工厂,能保证原料的高度一致性。排除植物在种植过程中受外界污染的可能:对比传统原料种植大都采用全开放式或半开放式的方式,我们的规模化种植将采取全封闭式、无土、无菌栽培,从源头上解决病虫害、动植物病毒、细菌、重金属和农药残留等问题。
    To solve the plant planting homogeneity problem: to establish an automated production line, combined with breeding technology and automated sowing, planting, irrigation and picking as one of the plant plant, to ensure a high degree of consistency of raw materials.
    Eliminate the possibility of contaminated plants in the process of planting: the traditional use of traditional raw materials are mostly open or semi-open way, our large-scale cultivation will be fully enclosed, soilless, aseptic cultivation, from the source to solve Pests and diseases, animal and plant viruses, bacteria, heavy metals and pesticide residues and other issues.

     

技术途径
Technical approach

将目标蛋白表达在植物种子中,人们可以通过种植此类植物来获得制造目标蛋白的原材料,为大规模生产和储运此类蛋白创造了条件。惠升将用此平台技术生产蚓激酶、阿替普酶 (t-PA)、替奈普酶(TNKase)、瑞替普酶(rt-PA)等溶血酶。

Expression of the target protein in plant seeds, people can plant such plants to obtain the target protein to produce raw materials for large-scale production and storage and transportation of such proteins to create the conditions. Hui Sheng will use this platform technology to produce lumbrokinase, alteplase (T-PA), TNKase, rituximab (rt-PA) and other hemolytic enzymes.

靶向烟草种子中的凝块溶解蛋白用以治疗中风
Targeting clonal protein in tobacco seedsUsed to treat stroke

比较优势
Comparative Advantage

  • 成本优势
    Cost advantage

    以阿替普酶(t-PA)为例,惠升生物采用专有平台技术,每亩转基因水稻可以生产出价值180 亿元的t-PA,供180万名急性缺血性脑卒中病人或者心血管病人使用。

    To alteplase (t-PA), for example, Hui Sheng biological using proprietary platform technology, per acre of transgenic rice can produce 18 billion yuan worth of t-PA, for 1.8 million patients with acute ischemic stroke or Cardiovascular patients use.

     
  • 储运优势
    Storage advantage

    以阿替普酶(t-PA)为例,转基因水稻种子易于保存和运输,储运要求大大降低。

    To alteplase (t-PA), for example, transgenic rice seeds easy to save and transport, storage and transportation requirements greatly reduced.